Purification and partial characterization of a Nocardia brasiliensis extracellular protease
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Partial Purification and Characterization of Protease Enzyme from Nomuraea Rileyi
Nomuraea rileyi (Farlow) Samson is an entomopathogenic fungus capable of producing a variety of enzymes including proteases and chitinases. Protease derived from microorganisms such as fungi, bacteria, and yeast has established wide spread applications in fields such as in the food, detergent and other industries. This study investigates the strategy for partial purification and characterizatio...
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The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
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Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...
متن کاملImmunogenicity and biophysical properties of a Nocardia brasiliensis protease involved in pathogenesis of mycetoma.
We isolated and purified to homogeneity a caseinolytic protease from a Nocardia brasiliensis cell extract. Preparative polyacrylamide gel electrophoresis and electroelution were employed for purification. This purified protease was injected in BALB/c mice and induced IgM and IgG anti-protease antibodies. Active immunization of mice with this protease prevented mycetoma development in experiment...
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1. Polasky, N., Polasky, S., Magenheim, H. & Abrams, N.R. Giant cell arteritis. Review and report of a case. JAMA 1965, 191: 341-343. 2. Nehra, P.C., Ramus, D.M. & Peterson, J. Giant-cell arteritis of the uterus with associated temporal arteritis: a case report. Obstet Gynecol 1990, 76: 935-938. 3. Padwell, A. Isolated arteritis of the uterine cervix. Three cases report. Br J Obstet Gynaecol 19...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1984
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.157.2.627-631.1984